Impact of Salinity and Light Intensity Stress on B Vitamins. Content in Marine Diatom Skeletonema costatum. Gede Suantika, Alissa Diany Putri. A.N. HandCarbohydrate metabolism in the marine diatom Skeletonema costatum Goor in statischer und homokontinuierlicher Kultur unter Phosphatlimitierung. Ammonium-Limited Continuous Culures of Skeletonema Costatum in Steady and I. Kultur und Synchronisation mit Entwicklungsstadien.

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Penelitian ini bertujuan untuk mengetahui dosis kascing yang baik pada Kultur sel Skeletonema costatum untuk menghasilkan kepadatan sel Skeletonema costatum yang optimal. Penelitian ini dilaksanakan pada tanggal 11 – 25 Desember Kultru penelitian yang digunakan adalah metode eksperiment dengan Rancangan Acak Lengkap RAL non faktorial yang terdiri dari 4 perlakuan dan 3 ulangan.

Assessment of the toxicity of sediment and seawater kulturr by the Prestige fuel spill using bioassays with clams Venerupis pullastra, Tappes decussatus and Venerupis rhomboideus and the microalga Skeletonema costatum ; Evaluacion de la toxicidad de sedimento y agua de mar contaminados por el vertido de fuel del Prestige, mediante el uso de bioensayos con las almejas Venerupis pullastra, Tappes decussatus y Venerupis rhomboideus y la microalga Skeletonema costatum.

In this study we present the early monitoring of the pollution caused by the accident of the Prestige tanker off the Galician coast. To evaluate the toxicity of sediment and water exposed to fuel, three kinds of bioassays were carried out using juvenile clams, clam embryos and microalgae.

Firstly, the burrowing behavior of Venerupis pullastra and Tappes decussatus in the sediment collected from two beaches of similar characteristics but different pollution conditions, was studied over 24 h.

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No significant differences were observed between the two sediments. Secondly, embryogenesis success of Venerupis rhomboideus was recorded after incubation in seawater and elutriates obtained from affected areas. In this case, fuel-polluted seawater showed a marked inhibition of embryogenesis, while sediment elutriates showed moderate toxicity. Parallel bioassays with the diatom Skeletonema costatum were carried out using sediment elutriates and filtered seawater from the sampling sites.

In the first sampling, significant differences were found in the response of S. Para evaluar la toxicidad del agua y el sedimento expuestos al fuel, se realizaron tres tipos de bioensayos: En los primeros, se evaluo el comportamiento de enterramiento de Venerupis pullastra y.

Feeding by phototrophic red-tide dinoflagellates on the ubiquitous marine diatom Skeletonema costatum. We investigated feeding by phototrophic red-tide dinoflagellates on the ubiquitous diatom Skeletonema costatum to explore whether dinoflagellates are able to feed on S. To explore effects of dinoflagellate predator size on ingestion rates of S. In addition, we measured ingestion rates of the common phototrophic dinoflagellates Prorocentrum micans and Gonyaulax polygramma on S.

We calculated grazing coefficients by combining field data on abundances of P. All phototrophic dinoflagellate predators tested i. Akashiwo sanguinea, Amphidinium carterae, Alexandrium catenella, Alexandrium tamarense, Cochlodinium polykrikoides, G. At the given prey concentrations, the maximum ingestion. Algal C and total carbon metabolisms 2.

Experimental observations with the diatom Skeletonema costatum. Three sets of comparisons of net and gross inorganic carbon assimilation and C uptake were made with an axenic culture of Skeletonema costatum.

Rate constants derived from the chemically determined changes were used to parameterize models that accounted for the respiration of photosynthetic products and for the recycling of respiratory CO The study has shown that we now possess the basis to make a rigorous analysis of net, gross CO2 fixation and net C Interfacial interactions between Skeletonema costatum extracellular organic matter and metal oxides: Implications for ceramic membrane filtration.

Lipids and proteins were also observed, although in lower ukltur. The current results contribute to advance our fundamental understanding of Algogenic Organic Matter AOM interfacial interactions with metal oxides i. In the current investigation, a rigorous characterization of the high molecular weight HMW compounds of Skeletonema costatum SKC intracellular organic matter IOMincluding nanomechanical properties, was conducted.

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Atomic force microscopy AFM provided crucial information of this isolate at a nanoscale resolution. Interestingly, two regions of different nanomechanical properties were observed: Uptake and depuration of I from labelled diatoms Skeletonema costatum to the edible periwinkle Littorina littorea.

Uptake and depuration of I into winkles through consumption of the diatom Skeletonema costatum is described.

The work follows on from previous studies that investigated the uptake of iodine into winkles from seawater and seaweed. Incorporation of I in S.

diatom skeletonema marinoi: Topics by

Iodine uptake in winkles from labelled S. This TF is lower than that observed for uptake of I in winkles from labelled seaweed. For the depuration stage, a biphasic sequence with biological half-lives of 1. The first phase is biokinetically important, given that winkles can lose two-thirds of kuptur activity during that period.

This study shows that, whilst winkles can obtain radioactive iodine from phytoplankton consumption, they do not retain the majority of that activity for very long. Hence, compared with other exposure pathways, such as uptake from seawater and macroalgae, incorporation from phytoplankton is a relatively minor exposure route.

Directory of Open Access Journals Sweden. Full Text Available Phosphorus P is a limiting macronutrient for diatom growth and productivity in the ocean. Much effort has been devoted to the physiological response of marine diatoms to ambient P change, however, the whole-genome molecular mechanisms are poorly understood.

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Here, we utilized RNA-Seq to compare the global gene expression patterns of a marine diatom Skeletonema skeletonemw grown in inorganic P-replete, P-deficient, and inorganic- and organic-P resupplied conditions. In total 34, unique genes were assembled and Genes participating in circadian rhythm regulation, such as circadian clock associated 1, were also up-regulated in P-deficient cells.

The response of S. This study provides new insights into the adaptive mechanisms to ambient P deficiency in marine diatoms. Ocean acidification OA and eutrophication intensifies in coastal sea under anthropogenic impact. OA coupled with the NH 4 -N source effect in coastal water is likely to affect the planktonic ecosystem.

In this work, Skeletonema costatum and Nitzschia closterium were chosen as typical species of diatom in Chinese coastal ecosystems to test the skeletonemw effect of OA and NH 4 -N. Results showed that the growth and NH 4 -N uptake of S. The maximum uptake rate is higher than the maximum growth rate, implying that NH 4 -N was assimilated faster for S.

Therefore, kultug inhibition rate of the growth of the two diatoms by the coupling effect of OA and eutrophication pH7. Consideration as Biodiesel Feedstock. Full Text Available Among the various potential sources of renewable energy, biofuels are of most interest.

Marine microalgae are the most promising oil sources for making biofuels, which can grow very rapidly and convert solar energy to chemical energy via CO2 fixation. The fatty acid profile of almost all the microalgal oil is suitable for the synthesis of biofuel. In this research, fatty acid and lipid contents of Bangladeshi strains of marine microalgae Skeletonema costatum were performed. For this, the crude oil was extracted by Soxhlet extraction method, using three most common solvent systems, pure hexane and mixture of CHCl3: Highest oil recovery It also indicated presence of hexadecatrienoic acid, benzenedicarboxylic acid, oleic acid, arachidonic acid, eicosapentaenoic acid EPA, 9-Octadecenoic acid methyl ester C19H36O2, and so forth.

The obtained fatty acid profile indicates high potentiality of S. Comparison between fluorimetry and oximetry techniques to measure photosynthesis in the diatom Skeletonema costatum cultivated under simulated seasonal conditions.

This study reports comparison of two techniques measuring photosynthesis in the ubiquitous diatom Skeletonema costatumi. Microalgae in semi-continuous cultures were exposed to five different environmental conditions simulating a seasonal effect with co-varying temperature, photoperiod and incident light.

Photosynthesis was assessed by gross rate of oxygen evolution P B and the electron transport rate ETR measurements. The light saturation intensity parameters Ek and Ek ETR ku,tur, and the maximum electron transport rate increased significantly with the progression of the season while the maximum light utilization efficiency for ETR alpha ETR was constant.


By contrast, the maximum gross oxygen photosynthetic capacity Pmax B and the maximum light utilization efficiency for P B alpha B increased from December to May treatment but decreased from May to July treatment. Both techniques showed clear photoacclimation in microalgae with the progression of the season, as illustrated by changes in photosynthetic parameters.


kultyr The relationship between the two techniques changed when high temperature, photoperiod and incident light were combined, possibly due to an overestimation of the PAR–averaged chlorophyll-specific absorption cross-section.

Despite this change, our results illustrate the strong suitability of in vivo chlorophyll fluorimetry to estimate primary production in the field. Karlodinium veneficum in India: Effect of fixatives on morphology and allelopathy in relation to Skeletonema costatum.

In this communication we provide a description of this species, possible methods for its identification in preserved samples and effect of its extracellular and intracellular extract Kultug significance of phytoplankton stickiness with emphasis on the diatom Skeletonema costatum.

Diatom aggregate formation was analyzed using coagulation theory. Population dynamics models show that coagulation has an important impact on species succession during diatom skeletlnema.

When different species collide and form mixed aggregates this process causes interspecific interference competitio Skeletonema Bacillariophyceae in Indian waters: The planktonic diatom genus Skeletonema is common in Indian coastal waters. Recent taxonomic studies have uncovered high diversity in this genus, and it is expected that several species occur also in the highly diverse marine habitats along Combined effects of pharmaceuticals, personal care products, biocides and organic contaminants on the growth of Skeletonema pseudocostatum.

Organisms in the environment are exposed to a number of pollutants from different compound groups. In addition to the classic pollutants like the polychlorinated biphenyls, polyaromatic hydrocarbons PAHsalkylphenols, biocides, etc.

Pharmaceuticals and personal care products PPCPs can be expected to co-occur with other organic contaminants like biocides, PAHs and alkylphenols in areas affected by wastewater, industrial effluents and intensive recreational activity.

In this study, representatives from these four different compound groups were tested individually and in mixtures in a growth inhibition assay with the marine algae Skeletonema pseudocostatum formerly Skeletonema costatum to determine whether the combined effects could be predicted by models for additive effects; the concentration addition CA and independent action IA prediction model.

The eleven tested compounds reduced the growth of S. Several binary mixtures and a mixture of eight compounds from the four different compound groups were tested. All tested mixtures were additive as model deviation ratios, the deviation between experimental and predicted effect concentrations, were within a factor of 2 from one or both prediction models e. Interestingly, a concentration dependent shift from IA to CA, potentially due to activation of similar toxicity pathways at higher concentrations, was observed for the mixture of eight compounds.

The combined effects of the multi-compound mixture were clearly additive and it should therefore be expected that PPCPs, biocides, PAHs and alkylphenols will collectively contribute to the risk in areas contaminated by such complex. Expression of death-related genes and reactive oxygen species production in Skeletonema tropicum upon exposure to the polyunsaturated aldehyde octadienal. ScDSP-1 and ScDSP-2 genes have been previously shown to be involved in cell death in ageing cells and in response to photosynthetic stress.

Interestingly, only intermediate levels of reactive oxygen species ROS i.